Cast[]
- Hank as Scooby Doo
- Theodore as Shaggy
- Foduck as Fred
- Emily as Daphne
- Carla as Velma
- Bedford as Scrappy Doo
- Policeman (from Thomas and Friends) as Police Officer
- Cabot as Phantom Virus
- Oliver as Ben Ravencroft
- Z-Stacks (from TUGS) as Various Villains
- Constance as Mrs. Cutler
- Nautilus as Scooby-Dum
- Blandford as Laurel
- Bingham as Hardy
- Fundy as Jerry Reed
- Northumberland as Uncle Nathaniel
- Captain Star (from TUGS) as The Mayor of Oakhaven
- Baddeck as Mr. McKnight
- Pugwash as Simone
- Shelburne as Omar
- Truro as Station Officer Wembley
- Phillip as Batman
- Fillmore as Robin
- Kulu as Louis
- Petra as Crystal
- Pearl as Amber
- George as Sheriff Bronson Stone
- Sigrid as Mayor Janet Nettles
- Stepney, Charlie, Billy and Rosie (from Thomas and Friends) as Sheriff Stone's Sons and Daughter
- Bluenose (from TUGS) as Mayor Jones
- Rebecca as Hot Dog Water
- Inverness as Barty Blake
- Lillie Lightship (from TUGS) as Nan Blake
- Digby as Dale Dinkley
- Madge (from Thomas and Friends) as Angie Dinkley
- Warrior (from TUGS) as Colton Rogers
- The Duchess (from TUGS) as Paula Rogers
- and more