- Wasabi as Spike (Wasabi can be Spike)
- Ikura as Mikey or Raphael
- Maguro as April
- Tako as Leo or Raphael
- Kani as Irma
- Ben the Man as Splinter
- Mochi as Karai
- Unagi as Kraang
- Fugu as Bebop
- Uni as Rocksteady
- Toro/Tuna as Slash (Toro can be Slash)
- Titanium Chef as Shredder