Vanity Smurf as Mario
Clumsy Smurf as Luigi
Pete (Mickey Mouse) as Wario
Mortimer Mouse as Waluigi
Pikachu as Yoshi
Buneary as Birdo
Smurfette as Peach
Smurfblossom as Daisy
Mother Nature as Rosalina
Gargamel as Bowser
Scruple as Bowser Jr
Tuck (Wonder Pets) as Larry Koopa
Brainy Smurf as Iggy Koopa
Jokey Smurf as Lemmy Koopa
Angelica Pickles as Wendy Koopa
Hefty Smurf as Morton Koopa
Snappy Smurfling as Toad
Sassette as Toadette