- Clarissa Saunders as Princess Peach
- Jefferson Smith as Mario
- Senator Joseph Harrison "Joe" Paine as Toadsworth
- Jim Taylor as Oogtar
- Governor Hubert "Happy" Hopper as Yoshi
- "Diz" Moore as Luigi
- Chick McGann as Toad
- Ma Smith as Princess Daisy
- Senate Majority Leader as Wario
- President of the Senate as Waluigi
- Susan Paine as Princess Rosalina
- Mrs. Hopper as Birdo
- Senator MacPherson as Bowser
- Senator Monroe as Bowser Jr.
- Senate Minority Leader as Donkey Kong
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