- Captain Marvel as Ms. Frizzle
- Goose as Liz
- Black Panther as Arnold
- Hawkeye as Ralphie
- Pepper Potts as Wanda
- Storm as Keesha
- Invisible Woman as Dorothy Ann
- Spider-Man as Carlos
- Falcon as Tim
- Mary Jane as Phoebe
- Shuri as Janet
- Black Widow as Dr. Tennelli
- Okoye as Tiffany
- The episodes "Works Out" and "Makes a Stink" have been cut.
- In the episode "Inside Hawkeye," all the references to Black Widow's role being Hawkeye's "mother" are cut.
- In the episode "Goes Cellular," the class do not go inside Mary Jane (Phoebe) first before going inside Black Panther (Arnold.) Instead, they go inside Falcon.
- The Quinjet replaces the bus.
- Jennifer Hale as Captain Marvel
- James C. Mathis III as the Black Panther
- John Reilly as Hawkeye
- Anna Cummer as Pepper Potts
- Iona Morris as Storm
- Lori Alan as the Invisible Woman
- Christopher Daniel Barnes as Spider-Man
- Deven Mack as the Falcon
- Sara Ballentine as Mary Jane
- Daisy Lightfoot as Shuri
- Vanessa Marshall as the Black Widow
- Yvette Nicole Brown as Okoye
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