- Coop Burtonburger as Mario
- Dennis as Yoshi
- Mr. Cheeks as Bowser Jr.
- Mr. Kat as Bowser
- Burt Burtonburger as Toadsworth
- Millie as Toadette
- Fiona as Peach
- Phoebe as Pauline
- Lorne as Wario
- Harley as Waluigi
- Young Coop as Baby Mario
- Young Dennis as Baby Luigi
- Young Lorne as Baby Wario
- Young Harley as Baby Waluigi
- Young Kat Kommander as Baby Bowser
- Miss Brannigan as Rosalina