Cast[]
- Ed as Nemo
- Eddy as Marlin
- Nazz as Dory
- Kevin as Gill
- Johnny as Bloat
- May Kanker as Peach
- Robot Jones (from Whatever Happened to Robot Jones?) as Gurgle
- Snap (from Chalkzone) as Bubbles
- Marie Kanker as Deb
- Lee Kanker as Flo
- Rolf as Jacques
- Edd as Nigel
- Gotta Sweep (from Baldi's Basics) as Crush
- 1st Prize (from Baldi's Basics) as Squirt
- Lord Hater (from Wander Over Yonder) as Phillip Sherman
- Sarah as Darla Sherman
- Eddy's Big Brother as Bruce
- The Yogman Twins (from Whatever Happened to Robot Jones?) as Anchor and Chum
- Plank as Blenny
- Wander (from Wander Over Yonder) as Mr. Ray
- Cuddles (from Happy Tree Friends) as Sheldon
- Toothy (from Happy Tree Friends) as Tad
- Giggles (from Happy Tree Friends) as Pearl
- Gordon, Edward and Henry (from Thomas and Friends) as Bob, Bill and Ted
- Molly Coddle (from Bump in the Night) as Coral
- Chinese Dragon (from Thomas and Friends) as Barracuda
- Old-Timey Ghost (from Homestar Runner) as Anglerfish
- Yonder Website Character Variations (from Homestar Runner) as The Seagulls
- King Bubsgonzola Supreme (from Homestar Runner) as The Whale