An Assist Trophy is an item, similar to a Poké Ball, that releases a non-playable character from a Nintendo game to assist the person who released them. Some of these characters are currently known.
| Character | Original series | Info |
|---|---|---|
| Hammer Bro. | Mario series | A Hammer Bro. from the Mario series throws his hammers at other players. |
| Lakitu | Mario series | Lakitu drops Spinies on other players. |
| Waluigi | Mario series | Waluigi stomps on enemy characters. |
| Kat and Ana | Wario series | These twin ninjas slash at players. |
| Tingle | Legend of Zelda series | Tingle summons a large group of random items. |
| Metroid | Metroid series | The titular Metroid flies around sucking the energy out of players. |
| Knuckle Joe | Kirby series | This enemy from the Kirby series attacks players with his fists and special moves. |
| Andross | StarFox series | The enemy of the StarFox team attacks players with polygonal shards fired from his mouth. |
| Samurai Goroh | F-Zero series | Captain Falcon's enemy appears to attack other players. |
| Lyn | Fire Emblem series | This hero from Fire Emblem attacks enemies with disappearing sword strikes. |
| Jeff | Earthbound series | Jeff launches a series of bottle rockets towards the stage. |
| Gray Fox | Metal Gear Solid series | Gray Fox attacks foes with a backhand swipe of his sword. He can also reflect projectiles. |
| Shadow | Sonic the Hedgehog series | This ally of Sonic's uses Chaos Control to slow down time. |
| Mr. Resetti | Animal Crossing series | The mole Mr. Resetti appears and lectures the player about Assist Trophies. His text bubbles fill the screen. |
| Nintendog | Nintendogs series | A Nintendog, from the DS series Nintendogs, appears and paws at the screen, blocking the players' views. |
| Dr. Wright | SimCity series | The obscure tutorial character from the original SimCity appears and raises buildings from the ground into other players. |
| Devil | Devil World series | This creature from the game Devil World, which was never released in the US, scrolls the screen in the direction he points. |
| Excitebike | Excitebike series | A group of Excitebike riders ride through the stage, running over players. |
| Tanks and Infantry | Advance Wars series | A series of Advance Wars fighters attack players. |
| Jill | Drill Dozer series | A Drill Dozer attacks other players. |
| Little Mac | Punch-Out!! series | Little Mac punches the other fighters. |
| Saki Amamiya | Sin and Punishment series | Saki shoots and slashes the enemy players. |
| Stafy | Legend of Stafy series | Stafy uses a spin attack on enemies. He is a rare example of an Assist Trophy that can be attacked. |
| Barbara | Daigasso Band Brothers series | Barbara plays damaging music. |
| Helirin | Kururin series | This Assist Trophy acts as a spinning platform. |
| Isaac | Golden Sun series | Isaac uses psyenergy push attacks on enemies. |
| Ray | Custom Robo series | Ray flies around and shoots energy blasts at foes. |
| Super Smash Bros. Brawl Official Site - Trailers | ||
| Playable Characters - Final Smash Attacks - Subspace Emissary - Stages - Items - Assist Trophies - Music - Included Franchises |