My Little Pony/The Rocky Horror Picture Show

Version 1 (SunsetShimmerFan)
Discord as Dr. Frank N Futer (a scientist)

Shining Armor as Brad (a hero)

Princess Cadence as Janet (a heroine)

Snips as Riff Raff (a handyman)

Demon Snips as Riff Raff in his space suit at the end of the film

Human Sunset Shimmer as Magenta (a domestic)

Demon Sunset Shimmer as Magenta in her space suit at the end of the film

Human Pinkie Pie as Columbia (a groupie)

Zecora as Dr. Scott (a rival scientist)

Human Flash Sentry as Rocky Horror (a creation)

Rainbow Dash as Eddie (ex-delivery boy)

Twilight Sparkle as The Criminologist (an expert)

Trixie as the Usherette

Princess Celestia as Betty

Prince Bluebood as Ralph