Thomas/The Life Bug

Thomas as Flik the Ant

Emily as Princess Atta

Rosie as The Queen

Isobella as Princess Dot

Duck as Thorny

Oliver as Cornelius

Edward as Mr. Soil

Mavis as Dr. Flora

Diesel as Hopper

Diesel 10 as Molt

D261 as Thumper

James as Francis

Percy as Heimlich

Daisy as Rosie

Toby as Slim

Gordon as Dim

Donald and Douglas as Tuck and Roll

Trevor as Manny

Molly as Gypsy

Sir Topham Hatt as P.T. Flea

Troublesame Trucks as Grasshoppers

Bill and Ben as Axel and Loco

Toby as Aphie the Aphid

Edward as Slick the Slug

Diesel 199, Hector and Dennis as The Fly Thud

Duke as The Ant Colony

Bertram as The Blueberries

Bertie as The Drummer