My Little Pony/The Incredibles

Twilight Sparkle as Bob Parr (Mr. Incredible)

Applejack as Helen Parr (Elastigirl)

Rainbow Dash as Dash Parr

Pinkie Pie as Violet Parr

Spike as Jack Jack Parr

Fluttershy as Lucius Best (Frozone)

Rarity as Edna E Mode

Discord as Buddy Pine (Syndrome)