Mario/Niki

Mario as Niki

Yoshi as Parodi

Luigi as Guglielmo

Peach as Margherita

Wario as Alberto

Waluigi as Lorenzo

Birdo as Benedetta

Daisy as Giulia

Rosalina as Laura

Toad as Bianchi

Toadette as Noemi

Toadsworth as Prof. Lombardi

Mallow as Spina

Pauline as Aurora

Bowser as Focacci

Lemmy "Hip" Koopa as Luca

Iggy "Hop" Koopa as Nicolò

Larry "Cheatsy" Koopa as Stefano

Wendy O. Koopa as Anna

Oogtar as Simone

Candy Kong as Beatrice

Tiny Kong as Lisbeth

Dixie Kong as Lorena