The Stationmaster's Wife/Isobella


 * The Stationmaster's Wife as Isobella
 * Judge Judy the Diesel as Lady
 * Smudger as Duck
 * Boulder as James
 * Oliver (from Theodore Tugboat) as Elizabeth
 * Shelly Marsh (from South Park) as Millie
 * Bulstrode as Old Slow Coach
 * Eric Cartman (from South Park) as Henry
 * Nelson Muntz (from The Simpsons) as Annie
 * Jimbo Jones (from The Simpsons) as Clarabel
 * Butters Stotch (from South Park) as Scruff
 * Lady Hatt as Spamcan (from RWS)
 * Zip (from TUGS) as Bash
 * Zug (from TUGS) as Dash
 * Zorran (from TUGS) as Gordon
 * Sir Topham Hatt as Judge Judy the Diesel
 * Elizabeth as Rusty
 * Farnsworth (from The Little Engine That Could) as Rosie
 * The Angry Policeman as Peter Sam
 * Paxton as Jeremy
 * Hector as BoCo
 * The Stationmaster as Kevin
 * Melvin Duck (from Skipper & Skeeto) as Max
 * Fungy Frog (from Skipper & Skeeto) as Monty
 * Taz-Mania Devil (from Taz Mania) as Rheneas
 * Splatter as Trevor
 * Dodge as Fergus
 * Norman as Byron
 * Sidney as Emily
 * The Diesel Shunter as Mavis
 * Harold as Hector
 * Patrick as Ferdinand
 * Dennis as Stephen
 * Zak (from TUGS) as Bill
 * Zebedee (from TUGS) as Ben
 * Diesel 10 as Jack
 * Vickie (from Shining Time Station) as Edward
 * Arthur as Evil Gordon
 * Skarloey as Evil Thomas
 * Rheneas as Evil Percy
 * Daisy as Lady Hatt
 * Diesel as Sir Topham Hatt
 * Ricky Tricky (from Skipper & Skeeto) as Harold
 * George as Skarloey
 * Mr. Conductor's Evil Twin (from Shining Time Station) as Whiff
 * D261 as Salty
 * Owen Olw (from Skipper & Skeeto) as George
 * Molly Mouse (from Skipper & Skeeto) as Thomas
 * Trevor as Bulgy
 * Conrad Cat (from Skipper & Skeeto) as Derek
 * Homer (from The Simpsons) as Farnsworth (from The Little Engine That Could)
 * Henrietta as Flora
 * Colin as Cranky
 * Luke as The Spiteful Breakvan
 * Mrs. Zero (from TUGS) as Mrs. Percival
 * Captain Zero (from TUGS) as Mr. Percival
 * Duncan as Terence
 * Carla (from Theodore Tugboat) as Stepney
 * Henry as Lorry 1
 * Bertram as Bertie
 * Constance (from Theodore Tugboat) as Hiro
 * Ruben Rabbit (from Skipper & Skeeto) as Scruffey
 * Den as Donald
 * Dart as Douglas
 * Vector (from Sonic) as Duncan
 * Annie as Lorry 2
 * Clarabel as Lorry 3
 * Bertie as Derek
 * Peter Sam as Dennis
 * Sir Handel as Frank (from RWS)
 * Whiff as Fire Cheif (from TUGS)
 * Flynn as Puffa (from TUGS)
 * Winston as Oliver
 * The Barber as Freddie
 * The Big City Engine (from RWS) as Flying Scotsman (from RWS)
 * The Duchess (from TUGS) as Georgia (from The Little Engine That Could)
 * Sarah (from Ed, Edd & Eddy) as Percy
 * Lorry 1 as O.J (from TUGS)
 * Lorry 3 as Billy Shoepack (from TUGS)
 * Bluenose (from TUGS) as Bluenose (from TUGS)
 * The Chinese Dragon as Owen (from Theodore Tugboat)
 * Lorry 2 as Old Rusty (from TUGS)
 * Bobby Barge (from Theodore Tugboat) as Ten Cents (from TUGS)
 * Sea Rouge (from Salty's Lighthouse) as Theodore (from Theodore Tugboat)
 * Sixteen (from RWS) as Shelburne (from Theodore Tugboat)
 * Bash as Green Eyed Pirate # 1 (from TUGS)
 * Dash as Green Eyed Pirate # 2 (from TUGS)
 * Old Stuck-Up (from RWS) as Zorran (from TUGS)
 * Flora as Sally Seaplane (from TUGS)
 * Moody Margaret (from Horrid Henry) as Skipper (from Skipper & Skeeto)
 * Horrid Henry (from Horrid Henry) as Molly Mouse (from Skipper & Skeeto)
 * Max as Mighty
 * Monty as Mac
 * Hector as Duke

Parodies

 * Bobby Barge/TUGS (The Stationmaster's Wife/Isobella version)
 * Sea Rouge (Salty's Lighthouse)/Theodore Tugboat (The Stationmaster's Wife/Isobella version)