Thomas/The Life's Bug

Thomas as Flik the Ant

Emily as Princess Atta

Elizabeth as The Queen

Rosie as Princess Dot

Duck as Thorny

Oliver as Cornelius

Edward as Mr. Soil

Mavis as Dr. Flora

Diesel as Hopper

Diesel 10 as Molt

Derek as Thumper

James as Francis

Percy as Heimlich

Daisy as Rosie

Toby as Slim

Gordon as Dim

Henry as Manny

Molly as Gypsy

Donald and Douglas as Tuck and Roll

Sir Topham Hatt as P.T. Flea

Trucks as The Fireflies

Troublesame Trucks as Grasshoppers

Bill and Ben as Axel and Loco

Trevor as Aphie the Aphid

Fergus as Slick the Slug

Duke as The Blueberries

Bertram as The Ant Colony

Bertie as The Drummer