Tanner&Friends/Pee-Wee Herman's Playhouse

Tanner (from Zelda) as Pee-Wee

Zelda the 2nd (from Zelda) as Doty

Snake (from Metal Gear Solid) as Cowboy Curtis

Samus (form Metroid) as Mrs.Evone

Jet (from Sonic) as Jambie

R.O.B (from Super Smash Bros Brawl) as Conky

Wavie (from Sonic) as Megic Screen

Koopas  (from Mario) as Flowers

Vio (from Zelda) as Randy