Scooby-Doo/Shining Time Station

Shaggy as Mr. Conductor (Ringo Starr)

Scooby Dum as Mr. Conductor (George Carlin)

Daphne as Stacy Jones

Scrappy Doo as Schemer

Scooby Doo as Matt

Velma as Tanya

Jerry Reed as Dan

Sybella as Kara

Lillie Lightship (from TUGS) as Becky

Fred as Harry Cupper

Detective Neville as Billy Twofeathers

The Mayor of Oakhaven as JB King

Ben Ravencroft as Mr. Conductor's Evil Twin

Mr. Beamon as Buster King

Phantom Virus as Hobart Hume

Lena as Vickie

Uncle Nathaniel as Mr. Nicholas

The Theodore Tugboat Characters as The Thomas Characters