My Little Pony Villains/Thomas

Cast (note: former villains are allowed to be counted as villains)

 * Princess Cadance as Thomas
 * Discord as Edward
 * Nightmare Moon as Henry
 * Tirek as Gordon
 * Chancellor Neighslay as James
 * Trixie as Percy
 * Grogar as Toby
 * Gilda as Duck
 * Flim as Donald
 * Flam as Douglas
 * Garble as Oliver
 * Twilight Sparkle as Diesel
 * Short Fuse as Bill
 * Rolling Thunder as Ben
 * Mane-iac as BoCo
 * Rarity as Daisy
 * Queen Chrysalis as Mavis
 * Gladmane as Stepney
 * Sunset Shimmer as Emily
 * Tatzlwurm as Bertie
 * Dr. Calebron as Salty
 * Wind Rider as Harvey
 * Applejack as Arry
 * Fluttershy as Bert
 * King Sombra as Fergus
 * Silver Fish (from Porky's Railroad) as Skarloey
 * Blue (from Old Tracks, New Tricks) as Rheneas
 * Hai Hai (from Kikansha Yaemon) as Sir Handel
 * Steve the Silver Engine (from The Little Engine That Won a Medal) as Peter Sam
 * Mark the Strong Engine (a Casey Jr. and Friends OC) as Rusty
 * Samson (from The Brave Locomotive) as Duncan
 * Cerberus (from The Little Engine That Could "2011 film") as Duke
 * Metro (from Rustee Rails Rides Again) as Freddie
 * The Haunted Train (from Hey Arnold) as Mighty Mac
 * Ahuizotl as Arthur
 * Daybreaker as Lady
 * Princess Celestia as Diesel 10
 * Spike as Splatter
 * Pinkie Pie as Dodge
 * Quarry Eel as Terence
 * Hydra as Trevor
 * Grubber as Toad
 * Storm King as Derek
 * Scootaloo as Bulgy
 * Apple Bloom as Elizabeth
 * Sweetie Belle as George
 * Suri Poliomare as Murdoch
 * Rainbow Dash as Spencer
 * Cozy Glow as Caroline
 * Derpy as Scruffey
 * Evil Rainbow Dash (from Secrets and Pies) as Harold
 * Tempest Shadow as Rosie
 * Background Ponies as Troublesome Trucks
 * Sunburst as Smudger
 * Shining Armor as Bulstrode
 * Jupiter Montage as Stanley
 * Evil Pinkie Pie (from Diana173076) as Molly
 * Diamond Tiara as Annie
 * Silver Spoon as Clarabel
 * Starlight Glimmer as The Spiteful Breakvan
 * Diesel 10 (from TTTE) as Jack
 * D261 (from TTTE) as Alfie
 * Diesel (from TTTE) as Oliver (Pack)
 * Snips as Max
 * Snails as Monty
 * D199 (from TTTE) as Kelly
 * Old Stuck Up (from TTTE) as Byron
 * Arry (from TTTE) as Ned
 * Bert (from TTTE) as Nelson
 * Splatter (from TTTE) as Buster
 * Dodge (from TTTE) as Isobella
 * Sixteen (from TTTE) as Patrick
 * Daisy, Lily Valley and Roseluck as The Horrid Lorries
 * Princess Luna as D261
 * Fume as Whiff
 * Clump as Scruff
 * Score as Dennis
 * Dumb Bell as Jeremy
 * Pony of Shadows as Billy
 * Rainbow Factory Dash as Belle
 * Pinkamena as Flynn
 * Spoiled Rich as Old Slow Coach
 * The Dragon (from Owl's Well That Ends Well) as Bash
 * The Dragon (from Dragonshy) as Dash
 * Hoops as Ferdinand
 * Vignette as Rocky
 * Yellow Engine (from Tiny Train and Mini Train) as Proteus
 * Lightning Dust as Hank
 * Tower (from The Little Engine That Could) as Colin
 * Smile HD Pinkie Pie as Charlie
 * Nightmare Rarity as Flora
 * Principal Cinch as Hector
 * Barker (from AICP Minneapolis 2007 and the Andrew Chesworth animation, Wild West) as Bertram
 * Equestria Girls Filthy Rich as Neville
 * Windigo as Captain
 * Lemon Zest as Den
 * Sour Sweet as Dart
 * Indigo Zap as Norman
 * Sunny Flare as Paxton
 * Sugarcoat as Sidney
 * Wallflower Blush as Madge
 * Cerberus as Butch
 * Descent as Hiro
 * Nightingale as Victor
 * Nightshade as Kevin
 * Crackle as Chinese Dragon
 * King Aspen as Thumper
 * Cockatrice as Henrietta
 * Babs Seed as Luke
 * Adagio Dazzle as Stephen
 * Aria Blaze as Caitlin
 * Sonata Dusk as Connor
 * Pegasus (from Wind in the Willows) as Millie