TV and Games/Despicable Me

Dr. Doofensmirtz (from phineas and ferb) as Gru

Mandark (from dexter's laboratory) as Vector

Goombas (from Mario) as Minions

Twilight Sparkle (from MLP: Friendship is magic) as Margo

Rainbow Dash (from MLP: Friendship is Magic) as Edith

Sweetie Belle (from MLP: Friendship is Magic) as Agnes