25 characters on one bicycle


 * 1) Twilight Sparkle (My Little Pony: Friendship is Magic)
 * 2) Pinkie Pie (My Little Pony: Friendship is Magic)
 * 3) Applejack (My Little Pony: Friendship is Magic)
 * 4) Rainbow Dash (My Little Pony: Friendship is Magic)
 * 5) Rarity (My Little Pony: Friendship is Magic)
 * 6) Fluttershy (My Little Pony: Friendship is Magic)
 * 7) Gary Coleman (Avenue Q)
 * 8) Horrid Henry
 * 9) Christmas Eve (Avenue Q)
 * 10) Dora the Explorer
 * 11) Jeff Dunham
 * 12) The Nostalgia Critic
 * 13) Pepper Ann
 * 14) Madeline
 * 15) Human Twilight Sparkle (My Little Pony: Equestria Girls)
 * 16) Human Pinkie Pie (My Little Pony: Equestria Girls)
 * 17) Human Applejack (My Little Pony: Equestria Girls)
 * 18) Human Rainbow Dash (My Little Pony: Equestria Girls)
 * 19) Human Rarity (My Little Pony: Equestria Girls)
 * 20) Human Fluttershy (My Little Pony: Equestria Girls)
 * 21) Blossom (The Powerpuff Girls)
 * 22) Bubbles (The Powerpuff Girls)
 * 23) Buttercup (The Powerpuff Girls)
 * 24) Miss Piggy (The Muppets)
 * 25) Stacy (Phineas and Ferb)