South Park/Dastardly and Muttley

Curly Goth as Dastardly

Vector (from Despicable Me) as Muttley

Scott (from The Little Engine That Could "2011 film") as Klunk

Stretch (from The Little Engine That Could "2011 film") as Zilly

Randy Marsh as Yankle Doodle Pigeon

Satan as The General